《自然-实验指南》（Nature Protocols）是一本由施普林格·自然（Springer Nature）出版的同行评审科学期刊。Nature Protocols is a peer-reviewed scientific journal published by Springer Nature. 该期刊专注于提供高质量、详尽的分步实验室操作指南与计算工作流程，主要涵盖生物学、化学及生物医学领域。The journal focuses on providing high-quality, detailed step-by-step laboratory recipes and computational workflows, primarily covering biological sciences, chemistry, and biomedicine.
《自然-实验指南》发表涵盖生物化学、细胞生物学、分子生物学、遗传学及计算生物学等领域的实验方案。Nature Protocols publishes experimental procedures across biochemistry, cell biology, molecular biology, genetics, and computational biology. 其发表的所有实验方案均采用循序渐进的格式编写，明确列出试剂配比、实验步骤、故障排除建议及预期实验结果。All published protocols are structured in a step-by-step format, clearly detailing reagent formulations, procedural stages, troubleshooting advice, and expected experimental results. 除湿实验方案外，该期刊还广泛收录用于生物数据分析及计算模拟的生物信息学流程。In addition to wet-lab protocols, the journal extensively covers bioinformatics pipelines used for biological data analysis and computational modeling. 大多数刊发的指南基于先前已发表并经过同行验证的研究技术，旨在提升科研结果的可重复性与透明度。Most published protocols build on previously published and peer-validated research techniques, aiming to enhance the reproducibility and transparency of scientific results.
该期刊于2006年6月由自然出版集团正式创刊，以补充《自然》系列研究期刊在实验细节上的不足。The journal was launched in June 2006 by Nature Publishing Group to complement the Nature research journals by providing comprehensive experimental details. 创刊初期，它还伴随建立了一个名为“Protocol Exchange”的开放式在线实验分享社区。At its launch, it was accompanied by an open online protocol-sharing community known as the Protocol Exchange. 随着施普林格与麦克米伦科学与教育于2015年合并，该期刊成为施普林格·自然旗下投资组合的一部分。Following the 2015 merger between Springer Science+Business Media and Macmillan Science and Education, the journal became part of the Springer Nature portfolio. 多年来，该刊已确立为生命科学与实验生物学领域最具影响力的实验方法学期刊之一。Over the years, it has established itself as one of the most prominent methodological journals in life sciences and experimental biology.
该期刊由受雇于施普林格·自然的专业全职编辑团队独立管理与运作。The journal is independently managed and operated by a team of professional, full-time editors employed by Springer Nature. 创刊主编为戴维·鲍鲍斯（David del Álamo），随后数位专业生命科学编辑相继主持编务工作。The founding chief editor was David del Álamo, followed by several professional life science editors leading editorial operations. 编辑团队的主要职责是评估来稿是否具备足够的创新性与可操作性，并组织严格的同行评审流程。The editorial team is responsible for evaluating incoming submissions for sufficient novelty and utility, as well as coordinating rigorous peer review processes. 此外，内部科学编辑还与作者密切合作，规范实验格式并完善实验排错指南，以确保技术细节的精确性。Additionally, in-house scientific editors work closely with authors to standardize protocol formats and refine troubleshooting sections, ensuring absolute technical accuracy.