Biology Methods and Protocols

Biology Methods and Protocols is an online-only open access journal from Oxford University Press that carries methodology and protocol papers across the biological sciences, founded in 2016 and holding a 2025 Journal Impact Factor of 2.4, a third-quartile place in Biochemical Research Methods.《生物学方法与实验方案》（Biology Methods and Protocols）是 Oxford University Press 出版的仅在线发行的开放获取期刊，刊登生物学各分支的方法与实验方案论文，2016 年创刊，2025 年期刊影响因子为 2.4，在生化研究方法类目居第三四分位。

Scope

• The remit is written plainly in the journal's own description, which names methodology and protocol papers in the biological sciences as the stock in trade.取材范围在刊物自述里写得直白，生物学的方法学与实验方案论文就是它的主营。

• Genetics and heredity, biochemistry, molecular biology and cell biology are the stated home fields, with ecology, evolutionary biology and marine and freshwater biology alongside them.自述点明的落脚处是遗传学与生物化学，分子生物学与细胞生物学并列，生态学、演化生物学和海洋与淡水生物学也在其中。

• Submissions are sorted into seventeen subject categories, running from biomolecular, structural and biophysical analysis through chromatin and epigenetics to transcriptome mapping.稿件按 17 个主题类目分流，从生物分子与结构与生物物理分析一路排到染色质与表观遗传学，末端是转录组作图。

• Under the structural heading the journal takes purification from awkward sources such as ancient DNA, quantification, and structural work by enzymatic assay, mass spectrometry, fluorescence, NMR or crystallography.结构方向的条目收下从古 DNA 这类难处理的来源提纯的方法，也收定量与结构解析的工作，手段涵盖酶学测定、凝胶电泳与质谱，荧光与核磁共振以及晶体学同样在列。

• Cell biology coverage reaches prokaryote and eukaryote processes, model single-cell organisms and their phenotyping, cell culture manipulation, stem cells, oncogenic processes and lab-on-a-chip systems.细胞生物学一侧覆盖原核与真核过程，模式单细胞生物及其表型分析在列，细胞培养操作与干细胞研究同收，肿瘤发生过程与芯片实验室系统也在范围内。

• Chromatin work is a category of its own, built around chromatin immunoprecipitation and other nucleosome and transcription factor mapping, together with the reading and inheritance of DNA and protein modifications.染色质与表观遗传学单列一类，核心是染色质免疫沉淀以及核小体与转录因子定位，DNA 与蛋白质修饰的读取和遗传传递一并归入。

• Computational methods cover programs, routines, metrics and algorithms for sequence manipulation, annotation and assembly, with an explicit demand that tools stay intelligible to non-specialists.计算方法收程序、例程与算法，序列操作、注释与组装都在其中，刊物明确要求工具对非专业读者也读得懂。

• Gene modification papers describe CRISPR, TALEN, zinc-finger nuclease and homing endonuclease platforms, peptide nucleic acid conjugates, and the delivery of nuclease, integrase or transposase activity to chosen loci.基因改造方向收 CRISPR 与 TALEN 平台，锌指核酸酶与归巢核酸内切酶同列，肽核酸偶联物也算，把核酸酶或整合酶活性送到指定基因座的做法一并收录。

• A separate category takes DNA-mediated cell transformation and nucleic acid transfer, including chemical modification and the upkeep of DNA, RNA or oligonucleotides inside cells and transgenic animals.DNA 介导的细胞转化与核酸导入另立一类，化学修饰在列，把 DNA、RNA 或寡核苷酸导入细胞与转基因动物并使其存续的做法同收。

• Genomics and polymorphism detection sit beside nucleic acid enzymology and the amplification and sequencing category, so a variant-calling or library-preparation method has a home without being forced into another field.基因组学与多态性检测同核酸酶学并列，扩增与测序类目紧邻其后，变体判定或文库构建的方法因此有处可放，不必硬塞进别的领域。

• Protein work spans recombinant expression and translation, protein-protein and protein-nucleic acid interaction, and proteomics, while RNA characterisation and manipulation keeps a heading of its own.蛋白质方向涵盖重组表达与翻译，蛋白质之间以及蛋白质与核酸的相互作用同收，蛋白质组学在列，RNA 表征与操作则单设标题。

• Synthetic biology and assembly cloning form one heading and immunological methodologies another, which keeps antibody development visible next to the molecular core rather than buried in it.合成生物学与组装克隆合成一个标题，免疫学方法另占一项，抗体与检测方法的开发因而紧挨着分子生物学这一主干，不会被埋没。

• Metagenomics and environmental biology carry the ecological side, matching the named interest in plant and animal ecology, evolutionary ecology, conservation ecology and theoretical ecology.宏基因组学与环境生物学承担生态那一侧，植物与动物生态学、演化生态学与保护生态学都在自述里点过名，理论生态学同样列入。

• Imaging technologies form a category of their own, a sign that detection and acquisition count as methods in the journal's accounting rather than as accessories to them.成像技术单列为类目，探测与采集在这份刊物的账上算方法，不算附属品。

• Software papers are courted directly, and the guidance is specific about what they must contain, namely a description of the program and one application of it.刊物专门征集软件类论文，要求写得具体，交代程序本身并给出一次实际应用。

• Every accepted manuscript has to present valid and reproducible results in enough detail for a reader to judge the inference and then run the method in a different laboratory.录用的稿件须给出有效且可重复的结果，细节要够读者判断推论是否成立，还要够他在另一间实验室把方法跑起来。

• Modifications of established methods are welcomed on the same footing as new ones, a position argued at launch with recombinant DNA as the example of small extensions that changed a field.对成熟方法的改良与新方法同等看待，这一立场在创刊时就摆了出来，重组 DNA 技术被用作例证，一串小延伸改变了整个领域。

History

• The journal opened in 2016, and the National Library of Medicine dates its holdings from volume 1, number 1, March 2016, carrying the title in PubMed and PubMed Central from that issue.刊物 2016 年问世，美国国家医学图书馆把馆藏起点定在第 1 卷第 1 期，即 2016 年 3 月，PubMed 与 PubMed Central 从这一期起收录。^[1]

• A launch editorial appeared on 29 February 2016 under the title BIOMAP: A Home for All Biology Methods, signed by Silvia Ayora of the Centro Nacional de Biotecnología in Madrid.创刊社论于 2016 年 2 月 29 日刊出，题为《BIOMAP，一切生物学方法的归宿》，署名者是马德里国家生物技术中心的（Silvia Ayora）。^[1]

• The case for a new title rested on scatter, since methods were being spread across journals in many categories and researchers in neighbouring fields met each other's techniques only by accident.办刊的理由建立在分散之上，方法论文散落在各类期刊里，相邻领域的研究者往往要碰巧才读得到同行手里的技术。

• Ayora argued that small steps count, pointing to recombinant DNA technology as a set of extensions to known procedures that turned out to be transformational in their effect.（Silvia Ayora）主张小步改进同样算数，她以重组 DNA 技术为例，说明那只是对已知流程的一串延伸，结果却改变了整个领域。^[1]

• Volume numbering has run one volume to a year without a break, so 2016 is volume 1, 2023 is volume 8, 2025 is volume 10 and 2026 is volume 11.卷号一年一卷，中间没有断档，2016 年为第 1 卷，2023 年为第 8 卷，2025 年为第 10 卷，2026 年为第 11 卷。

• Each volume carries a single issue, numbered 1, and articles are identified by article numbers rather than by page ranges, so a paper is cited by its own string.每卷只出一期，期号恒为 1，文章以文章编号标识，不用起止页码，引用时直接给那串编号。

• Access was open from the first issue under a Creative Commons Attribution Non-Commercial licence, and the same CC BY-NC terms still govern papers published in 2024 and 2025.自第 1 期起刊物就按知识共享署名非商业性使用许可开放，2024 年与 2025 年的论文仍沿用 CC BY-NC 条款。

• Because it launched online only, there is no print run and no mailed issue, and accepted manuscripts go up as advance articles before being folded into the volume.刊物自始仅在线出版，没有印本，也不寄送期次，录用稿先以预出版文章上网，随后才归入当年那一卷。

• Coverage in the Web of Science sits in the Emerging Sources Citation Index rather than the Science Citation Index Expanded, and the citation category is Biochemical Research Methods.在 Web of Science 里，刊物归入新兴来源引文索引，尚未进入科学引文索引扩展版，计量类目为生化研究方法。

• The 2025 report gives a Journal Impact Factor of 2.4 and a five-year figure of 2.9, with 783 citations, 86 items and a cited half-life of 3.1 years.2025 年的报告给出影响因子 2.4，五年影响因子 2.9，总被引 783 次，该年刊出 86 篇，被引半衰期 3.1 年。

• That value puts the title at rank 55 of 85 in its category, the third quartile and the 35.9th percentile, after a 2023 figure of 2.5 and a dip to 1.3 in 2024.该数值把刊物放在类目 85 种中的第 55 位，属第三四分位，百分位 35.9，此前 2023 年为 2.5，2024 年落到 1.3。

• Open access is complete rather than partial, and the 2025 report records 100 percent of content as gold, which matches the licence line printed on every article examined.开放获取是全覆盖，2025 年报告把全部内容记为金色开放获取，与逐篇核查所见到的许可行一致。

• The cover and the National Library record the title with an ampersand, Biology Methods & Protocols, while Crossref and OpenAlex register it with and, and citations use both forms.封面与国家医学图书馆的著录都以 & 连接，写作 Biology Methods & Protocols，Crossref 与 OpenAlex 则记作 Biology Methods and Protocols，引用中两种写法并存。

Editors

• Andrzej Stasiak of the University of Lausanne in Switzerland holds the title of Editor in Chief, a post he already held in the 2020 and 2022 versions of the board listing.主编为洛桑大学的（Andrzej Stasiak），2020 年与 2022 年的编委名录都把他列在这一职位上，任期延续至今。

• Silvia Ayora, who signed the launch editorial as Editor in Chief, later moved to an associate editor role and is listed with the Spanish National Center for Biotechnology in Madrid.（Silvia Ayora）以主编身份署名创刊社论，后来转任副编辑，名录上的单位写的是马德里西班牙国家生物技术中心。

• Three further associate editors complete the handling tier, Hodaka Fujii at Hirosaki University in Japan, Florencio Pazos at the Centro Nacional de Biotecnología and Dagmar Wirth at the Helmholtz Centre for Infection Research.副编辑一层另有三人，弘前大学的（Hodaka Fujii）来自日本，国家生物技术中心的（Florencio Pazos）与不伦瑞克亥姆霍兹感染研究中心的（Dagmar Wirth）分列其后。

• Counting the editor in chief and the four associate editors with the board, the editorial team runs to thirty-one people across twelve countries, Britain supplying eight, Spain six, the United States five, Germany three and Japan two.把主编与四位副编辑同编委一并计算，编辑队伍共 31 人，分布在 12 个国家，其中英国 8 位，西班牙 6 位，美国 5 位，德国 3 位，日本 2 位。

• Smaller representations come from Australia, France, Poland, Singapore, China, Tanzania and Switzerland, so the centre of gravity is European without the board being European only.澳大利亚、法国、波兰、新加坡、中国、坦桑尼亚与瑞士各占一席，编委的重心在欧洲，覆盖面却不止于欧洲。

• Sue Hedges is named as managing editor for editorial and production, the one post on the page that is not a research appointment.编辑与生产一栏由（Sue Hedges）任编辑主任，编委页上只有这一职位不带研究教席。

• Review is described as thorough and follows the practice of other Oxford University Press journals, and the launch editorial promised that accepted manuscripts would be visible online in the shortest possible time.评审被描述为严格，沿袭 Oxford University Press 其他刊物的做法，创刊社论还承诺录用稿会以最短时间上线。

• Board members were chosen for having contributed across different branches of biology, which is how the claim to be interdisciplinary turns into an appointment list.编委的遴选看的是在生物学不同分支里都有建树，跨学科的主张因此在人事安排上落了地。

• Software submissions carry an explicit condition that the code be released where that is possible, and the editors treat the program as part of the method.软件类稿件有一条明示条件，代码在可行时须公开，编辑把这段程序算作方法本身的一部分。

• For every paper the test is the same, whether a reader can follow the description closely enough to repeat the work, and manuscripts that stop short are sent back for detail.全部稿件用同一把尺子衡量，读者能否照描述把工作重做一遍，细节不足的稿件会被退回补充。

• The board reaches into industry and applied research through posts at Novartis and at CABI, which fits a remit running from bench protocols to field and clinical measurement.编委里有人在 Novartis 与 CABI 任职，产业与应用研究的席位由此出现，与从实验台方案延伸到田间和临床测量的取材范围相合。